Electrophoresis Parameters Sample Clauses

Electrophoresis Parameters. Using the instructions provided in the Operator’s Manual, set up parameters as follows: * Due to variation in environmental conditions, a Blot 1 time of 3 minutes is recommended, but a range of 2 to 5 minutes is acceptable. Separator Unit • Serum (Blade Application) Load Sample Prompt: None Time: 0:30 Temperature: 21°C Speed: 6 Apply Sample Prompt: None Time: 0:30 Temperature: 21°C Speed: 1 Location: 1 Electrophoresis Prompt: None Time: 7:00 Temperature: 21°C Voltage: 650 V mA: 160 mA Absorb Prompt: Remove Gel Blocks, Apply Antisera Time: 2:00 Temperature: 21°C Blot 1 Prompt: Remove Excess Antisera Time: 3:00* Temperature: 21°C Blot 2 Prompt: Remove Template, Install Blotter Time: 5:00 Temperature: 40°C Dry Prompt: Remove Blotter Time: 15:00 Temperature: 50°C End • Urine or Urine and Serum (Blade Application) Load Sample 1 Prompt: None Time: 0:25 Temperature: 21°C Speed: 6 Apply Sample 1 Prompt: None Time: 0:25 Temperature: 21°C Speed: 6 Location: 1 Load Sample 2 Prompt: None Time: 0:25 Temperature: 21°C Speed: 6 Apply Sample 2 Prompt: None Time: 0:25 Temperature: 21°C Speed: 6 Location: 1 Load Sample 3 Prompt: None Time: 0:25 Temperature: 21°C Speed: 6 Apply Sample 3 Prompt: None Time: 0:25 Temperature: 21°C Speed: 6 Location: 1 Absorb 1 Prompt: None Time: 2:00 Temperature: 21°C Electrophoresis Prompt: None Time: 7:00 Temperature: 21°C Voltage: 650 V mA: 160 mA Absorb Prompt: Remove Gel Blocks, Apply Antisera Time: 2:00 Temperature: 21°C Blot 1 Prompt: Remove Excess Antisera Time: 3:00* Temperature: 21°C Blot 2 Prompt: Remove Template, Install Blotter Time: 5:00 Temperature: 40°C Dry Prompt: Remove Blotter Time: 15:00 Temperature: 50°C End • Urine or CSF (Template Application) Pause Prompt: None Time: 5:00 Temperature: 21°C Electrophoresis Prompt: To Continue Time: 7:00 Temperature: 21°C Voltage: 650 V mA: 160 mA Absorb Prompt: Remove Gel Blocks, Apply Antisera Time: 2:00 Temperature: 21°C Blot 1 Prompt: Remove Excess Antisera Time: 3:00* Temperature: 21°C Blot 2 Prompt: Remove Template, Install Blotter Time: 5:00 Temperature: 40°C Dry Prompt: Remove Blotter Time: 15:00 Temperature: 50°C End Stainer Unit • Serum, CSF or Urine (Both Application Methods)
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Electrophoresis Parameters. A. Serum
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Electrophoresis Parameters. Using the instructions provided in the appropriate Operator’s Manual, set up parameters as follows for the SPIFE 3000: Electrophoresis Unit
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