Immunogold material Sample Clauses

Immunogold material. Five blocks of sriatal tissue from 5 animals were cut out from slides, mounted on resin blocks, and glued into place using cyanoacrylate glue. Blocks were collected in the same regions of striatum as those taken for immunoperoxidase observations. Ultrathin sections from the surface of the blocks were examined under the electron microscope, and randomly selected elements containing gold particles were digitized at high (25,000×) magnifications. The immunogold method was paired with the immunoperoxidase technique in order to immunolabel two different antigens. This double labeling technique was applied to the vGluT1 and vGluT2 colocalization experiment as well as the group III mGluR and D1 receptor assay.
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