In Vitro ADCC and NK Observations Sample Clauses

In Vitro ADCC and NK Observations. To characterize the potential for VTX-2337 to modulate immune function beyond the characterization in PD studies, immunophenotyping of peripheral blood and NK cell cytotoxic activity was assessed. These in vivo studies demonstrated that administration of VTX-2337 results in transient changes in blood cell populations consistent with cellular activation of neutrophils, NK cells, and T cells and may result in the transient extravasation of these latter cell populations from the blood compartment.[16] In addition, VTX-2337 stimulates the production of cytokines from mDC, monocytes, and NK cells in vitro.[17] VTX-2337 stimulates ADCC when used in combination with a variety of monoclonal antibodies of the IgG1 subclass including rituximab and traztuzumab.[17] More recently, in collaboration with Xx. Xxxxxx Xxxxxx at the University of Pittsburgh, it was demonstrated that VTX-2337 increases the cetuximab-mediated killing of SCCHN targets in an NK-dependent manner.[18] The TLR8 and NK-dependent enhancement of ADCC occurs in samples from patients of all three FcRγ3a genotypes, including those with the allele encoding the homozygous F/F variant that is associated with lower affinity for the Fc receptor. In addition, the stimulation of mDC and monocytes concurrent with cetuximab administration was tested to determine the promotion of development of EGFR-specific T cells via a mechanism referred to as ‘cross-priming’. Using a mixed culture model in vitro, tetramer analysis (using a molecular reagent that detects human leucocyte antigen-specific, EGFR peptide specific CD8+ T cells) demonstrated that VTX-2337 stimulates cross-priming and increases the number of EGFR-specific CD8+ T cells following cetuximab treatment. Taken together, the ability of VTX-2337 to stimulate the production of inflammatory cytokines (including XX-0, XX-00, XXXx, XX-00, and IFNg), the activation and stimulation of NK cells and ADCC, and the activation of the antigen processing and presentation of mDC and monocytes provide a strong rationale that this TLR8 agonist may enhance the activity of cetuximab in clinical studies.
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Related to In Vitro ADCC and NK Observations

  • Informal Observations A. An informal observation is an observation that is not required to be pre-scheduled.

  • Unannounced Observations i) Unannounced observations may be in the form of partial or full-period classroom visitations of not less than ten minutes duration.

  • Formal Observations 1. A minimum of two (2) formal observations shall be conducted to support the final summative evaluation. All formal observations shall last a minimum of thirty (30) consecutive minutes and will take place between September 15 and April 30. There will be a minimum of fifteen (15) work days between the first two (2) formal observations.

  • Classroom Observation Classroom observations shall be conducted at least once for all unit members who have not met the threshold for reappointment as defined in Section 10.02 of Article10. Effective fall 2006 classroom observations shall be conducted on DCE form E3. Classroom observations may be conducted for any unit member, at the discretion of the President of the College, or designee thereof, for stated written reasons. Results of said observation shall be communicated to the unit member in writing. The unit member shall be afforded the opportunity to discuss the classroom observation with the evaluator and to respond in writing.

  • Informal Observation Additional evaluation of employees may occur through informal observations by the Employer. It may not be necessary to reduce such evaluations to writing, and a verbal discussion between the Employer and the employee concerning the informal observations may suffice. If a written evaluation is deemed necessary, Procedures C and D as outlined above shall be followed.

  • Observations The Evaluator’s first observation of the Educator should take place by November 15. Observations required by the Educator Plan should be completed by May 15th. The Evaluator may conduct additional observations after this date. The Evaluator is not required nor expected to review all the indicators in a rubric during an observation.

  • Adverse Events Subsequent to the date hereof, there shall not have occurred any of the following: (i) a suspension or material limitation in trading in securities generally on the New York Stock Exchange, the NASDAQ National Market or the NASDAQ Global Market, (ii) a general moratorium on commercial banking activities in the People’s Republic of China or New York, (iii) the outbreak or escalation of hostilities involving the United States or the People’s Republic of China or the declaration by the United States or the People’s Republic of China of a national emergency or war if the effect of any such event specified in this clause (iii) in your reasonable judgment makes it impracticable or inadvisable to proceed with the public offering or the delivery of the Shares on the terms and in the manner contemplated in the Prospectus, or (iv) such a material adverse change in general economic, political, financial or international conditions affecting financial markets in the United States or the People’s Republic of China having a material adverse impact on trading prices of securities in general, as, in your reasonable judgment, makes it impracticable or inadvisable to proceed with the public offering of the Shares or the delivery of the Shares on the terms and in the manner contemplated in the Prospectus.

  • Pre-Observation Conference The pre-observation conference shall be held prior to each formal observation. The teacher and evaluator will mutually agree when to conference. The purpose of the pre-observation conference is to discuss the employee’s goals, establish a date for the formal observation, and to discuss such matters as the professional activities to be observed, their content, objectives, strategies, and possible observable evidence to meet the scoring criteria.

  • Formal Observation The instructional employee and the principal/administrator shall review using the mid-year Reflection Questions in the evaluation manual – Student Achievement Outcomes and make adjustments as needed to increase the likelihood of a positive goal outcome.

  • Additional Information for Product Development Projects Outcome of product development efforts, such copyrights and license agreements. • Units sold or projected to be sold in California and outside of California. • Total annual sales or projected annual sales (in dollars) of products developed under the Agreement. • Investment dollars/follow-on private funding as a result of Energy Commission funding. • Patent numbers and applications, along with dates and brief descriptions.  Additional Information for Product Demonstrations: • Outcome of demonstrations and status of technology. • Number of similar installations. • Jobs created/retained as a result of the Agreement.

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