Luminescence Sample Clauses

Luminescence. Luminescent techniques can be used to measure the emission of light as a result of ROS generation. For example, 1O2 has one electron at a higher energy state and emits the surplus energy as light when relaxes to its ground state57. Although luminescence can be used to measure 1O2 on a cellular level, its application for quantifying 1O2 in intact tissues in vivo is hampered by light absorption, scatter, and the very low levels of light emitted88. The chemiluminescence probe lucigenin is specific for detecting ∙O -. Lucigenin is reduced by ∙O - to form a lucigenin radical. The lucigenin radical reacts with a second ∙O - to generate an energy rich dioxetane molecule which then emits light84. Although lucigenin is specific for ∙O -, the lucigenin radical produced upon its reduction is capable of reacting with molecular oxygen to create further ∙O -. Xxxxxxxxx could therefore overestimate ROS generation.
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