Principles of ELISA Sample Clauses

Principles of ELISA. Principle of ELISA (Anti syphilis AbDialab) The Anti-Syphilis Ab ELISA is a solid phase qualitative enzyme immunoassay based on sandwich principle for the detection of total antibodies to Treponemapallidumin human serum or plasma. The micro plate is coated with recombinant antigens for T pallidum. During testing, the specimen and the enzyme conjugated T. pallidum antigens are added to the antigen coated micro well plate and then incubated. If the specimen contain antibodies to T. pallidum, it will bind to the antigens coated on the micro well plate and simultaneously bind to the conjugated immobilized antigen T. pallidum conjugate complexes. If the specimen does not contain antibodies to T. pallidum the complex will not be formed. After initial incubation, the micro well plate is washed to remove unbound materials. Substrate A and substrate B are added and then incubated to produce a blue color indicating the amount of T. pallidum antibodies present in the specimen Sulphuric acid solution is added to the micro well plate to stop the reaction producing a color change from blue to yellow. The color intensity which corresponds to the amount of T. pallidum antibodies present in the specimen is measured with a micro plate reader at 450/630 -700 nm or at 450 nm. (kit insert of Dialab syphilis).
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