Propagation of seed Sample Clauses

Propagation of seed. Seed found on the in situ plant (aka: Mother Plant) and from the seed bank in the soil may be of two types, self-fertile or cross pollinated by another A. franciscana or a closely related manzanita. If self-fertile, there would be no additional genetic material in the seedling. However, due to reduction and recombination of chromosomes during meiosis, different genes are paired producing a genotypically unique individual. If cross-pollinated by pollen from another individual A. franciscana or closely related species, new genetic material would have been introduced and would produce a genetically unique individual. Since there are presently no other A. franciscana nearby (within pollinator travel distance-usually bees) (unless the in situ plant is actually different individuals) it is most likely, that the seeds collected are self-fertilized. Any seedlings germinating from seeds from the mother plant or seed bank should undergo DNA sequencing to determine degree of heterozygosity, whether they are recombined offspring of the one plant or have unique genetic characters from another plant. This would be done 1-5 years after germination, when the seedling plants have reached sufficient size. (Presidio Trust) Seed from the summer seed set on the mother plant in 2009 were collected from the plant. Approximately 300 seeds were collected. Taking the most conservative approach, the assumption must be made that the in situ plant does not survive translocation, and therefore the seeds that are collected from the plant and seed bank are the only non-clonal propagules that would remain from this plant. Seed collected should be divided in half. One half of the seed should be sent to long-term seed conservation storage facility at Rancho Santa Xxx Botanic Gardens and the other half to UC Berkeley Botanical Garden or to another facility as deemed appropriate by the agencies. See Long Term portion of the plan for further information. (Caltrans) There is no definitive method for successful germination of this species. Success rates in botanical gardens have been 1-2%. Experiments should be carried out on closely related species such as A. hookeri and A. montana until a definitive propagation protocol is perfected. Only then should an attempt be made to germinate either the seed collected from the plant itself or seed in the soil seed bank. The following may inform germination trials on closely related species before germination of seed in the seed bank soil is attempted. On...
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