Protein Assay Sample Clauses

Protein Assay. A standard curve was constructed using a protein standard (bovine gamma-globulin standard) between the concentration range of 0.32 and 1.32 mg/ml. The standards (5 µl) were pipetted into a 96-well plate (6 replicates for each concentration) followed by 25 µl of the reagent A (provided with the kit) and then followed by 200 µl of reagent B. The plate was agitated to mix the reagents. The same procedure was applied to the samples obtained from the cell lysis step of the transfection experiment. After 15 min, absorbance of both the standards and the samples were read at 750 nm using the SpectraMax 190 Microplate Spectrophotometer (Molecular Devices, USA). The amount of protein expressed by the cells was determined from the calibration curve (Appendix).
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Related to Protein Assay

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