Real-time PCR Sample Clauses

Real-time PCR. In order to quantify the amount of DPL that had trafficked from the graft to the DLN of the recipient, a real-time PCR assay for a donor-specific gene was used. To calculate the copy number of the donor-specific gene from unknown samples, a mean was calculated from triplicate ct values, applied to a standard curve generated from donor strain DNA, and corrected for the total amount of DNA in the sample.
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Related to Real-time PCR

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