Sample Template Application Sample Clauses

Sample Template Application. 1. Carefully cut open one end of the gel pouch. Remove one gel from the protective packaging. Fold and tape the open end of the pouch to prevent drying of the remaining gel. Remove the gel to be used from the plastic mold and discard the mold. 2. Dispense approximately 1 mL of REP Prep onto the left side of the electrophoresis chamber. 3. Place the left notch of the gel so that it fits the left pin of the chamber floor and gently roll the gel to the right side fitting the right notch to the right pin of the chamber floor. Use a lint free tissue to wipe around the edges of the gel backing to remove any excess REP Prep. Make sure that no bubbles remain under the gel. 4. Using a QuickGel Blotter C, gently blot the entire gel using slight fingertip pressure on the blotter. Remove the blotter. 5. Remove one QuickGel Template from the package. Hold the template so that the small hole in the corner is toward the front right side of the chamber. 6. Carefully place the template on the gel aligning the template slits with the marks on each side of the gel backing. The center hole in the template should align with the indention in the center of the gel. 7. Apply slight fingertip pressure to the template making sure there are no bubbles under it.
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Sample Template Application. 1. Carefully cut open one end of the gel pouch. Remove one gel from the protective packaging. Fold and tape the open end of the pouch to prevent drying of the remaining gel. Remove the gel to be used from the plastic mold and discard the mold. 2. Dispense approximately 1 mL of REP Prep onto the left side of the electrophoresis chamber. 3. Place the left notch of the gel so that it fits the left pin of the chamber floor and gently roll the gel to the right side fitting the right notch to the right pin of the chamber floor. Use a lint free tissue to wipe around the edges of the gel backing to remove any excess REP Prep. Make sure that no bubbles remain under the gel. 4. Using a QuickGel Blotter C, gently blot the entire gel using slight fingertip pressure on the blotter. Remove the blotter. 5. Remove one QuickGel Template from the package if only one row of samples is tested. Remove two templates if 11 to 20 samples are tested. Hold the template so that the small hole in the corner is toward the front right side of the chamber. 6. Carefully place the template on the gel aligning the template slits with the marks on each side of the gel backing. The center hole in the template should align with the indention in the center of the gel. 7. Apply slight fingertip pressure to the template making sure there are no bubbles under it. NOTE: If wearing rubber gloves to perform this step, place a QuickGel Blotter A over the template and then apply fingertip pressure to the template. Remove the blotter. Powder from the gloves can produce gel artifacts. 8. Apply 2 µL of the appropriate sample to the template slits. After the last sample application, wait 3 minutes to allow time for the proper absorption. 9. Use the QuickGel Blotter A to gently blot the excess sample from the template. Carefully remove the blotter and template and dispose of them as biohazardous waste. 10. Close the lid, press the power switch to turn on the chamber and start the power supply.

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