The LDH assay Sample Clauses

The LDH assay. In healthy cells, LDH is located exclusively within the cytosol. However, during cell death, plasma membrane rupture leads to LDH leakage into the surrounding media. By measuring the amount of LDH that has been released from cells, the LDH assay reveals the extent of plasma membrane rupture and as such a measure of late- stage toxicity and cell death (Xxx & Xxxx 1987). In practice, LDH presence in the media is determined via the measurement of the downstream production of a red formazan dye (figure 3.1). Lactate Formazan Lactate dehydrogenase (LDH) NAD+ Diaphorase NADH Pyruvate Iodonitrotetrazolium
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