Trypan Blue Assay Sample Clauses

Trypan Blue Assay. Following treatment with radiolabelled lipophilic complexes as described in Section 3.3.3.4, 2 x 105 cells were seeded in 6-well plates, with 2 mL per well of complete media and left to grow for 72 hours at 37°C in 5% CO2 incubator. The supernatant was then removed and cells were washed twice with DPBS, trypsinised and counted for viability with trypan blue with number of viable cells were counted and represented in percentage relative to the untreated group.
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