Examples of Figure 6 in a sentence
Prepare an intermediate dilution series of each test compound by 4X serial dilution in DMSO such that the top concentration is 1 mM (suggested starting point) (Figure 6, Step 1).
Add 5 µL of each concentration of serially diluted compound to triplicate assay wells in a 384-well plate (columns 1-3) as depicted in Steps 2 and 3 of Figure 6.
Continue process as depicted in Step 1 of Figure 6.Column 1Column 2Column 1Column 2Column 1Column 2 STEP 1 STEP 2 STEP 3 STEP 4 A B C D E Figure 6.
Remove 5 µL of each concentration of diluted compound, transfer to another 96-well plate, add 162 µL of Kinase Buffer A and mix (Figure 6, Step 2).3.
LC50 Median Lethal Concentration (Determined at 48 Hours) Methods of Estimation:• Probit Method• Spearman-Karber• Trimmed Spearman-Karber• Graphical See the flow chart in Figure 6 on p.