Examples of Hot Start in a sentence
Denaturation: Phusion Hot Start Flex DNA Polymerase does not require a separate activation step.An initial denaturation of 30 seconds at 98°C is sufficient for most amplicons from pure DNA templates.
It is important to note thatif a high concentration of DMSO is used, the annealing temperature must be lowered as DMSO decreases the primer Tm (2).Phusion Hot Start Flex DNA Polymerase is also compatible with other additives such as formamide or glycerol.
Phusion Hot Start Flex DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates.Each of these buffers contains MgCl2 (1.5 mM at the final [1X] reaction concentration).
Commer- cial use of the aptamer-based Phusion Hot Start Flex DNA Polymerase requires a license from New England Biolabs, Inc.
The additional commitment and inspection fees shall be paid within 30 days after the date that the increase is granted.§ 242.20 Reopening of expired commit- ments.An expired commitment may be re- opened if a request for reopening is re- ceived by HUD no later than 90 days after the date of expiration of the com- mitment.