Examples of Hot Start in a sentence
The final concentration of each primer in a PCR experiment using Phusion Hot Start Flex DNA Polymerase may be 0.2–1 µM, while 0.5 µM is strongly recommended.
We generally recommend using Phusion Hot Start Flex DNA Polymerase at a concentra- tion of 20 units/ml (1.0 unit/50 µl reaction).
Phusion Hot Start Flex cannot incorporate dUTP and is not recommended for use with uracil-containing primers or template.
However, the optimal concentration of Phusion Hot Start Flex DNA Polymerase may vary from 10–40 units/ml (0.5–2 units/50 µl reaction) depending on amplicon length and difficulty.
PCR was per- formed using Q5 Hot Start High-Fidelity 2X Master Mix (NEB # M0494) and Illumina small RNA primers RP1 and RPl1 (100ng oligos, 2.5ul of each primer (10µM), 25ul Q5 Master Mix in a 50ul reaction).