Examples of Hot Start in a sentence
The additional commitment and inspection fees shall be paid within 30 days after the date that the increase is granted.§ 242.20 Reopening of expired commit- ments.An expired commitment may be re- opened if a request for reopening is re- ceived by HUD no later than 90 days after the date of expiration of the com- mitment.
However, the optimal concentration of Phusion Hot Start Flex DNA Polymerase may vary from 10–40 units/ml (0.5–2 units/50 µl reaction) depending on amplicon length and difficulty.
Phusion Hot Start Flex cannot incorporate dUTP and is not recommended for use with uracil-containing primers or template.
Indicative answers, which were given by the trainees, are shown.• Short comments on the answers are provided, by interpreting meaning from the content of text data.
PCR was per- formed using Q5 Hot Start High-Fidelity 2X Master Mix (NEB # M0494) and Illumina small RNA primers RP1 and RPl1 (100ng oligos, 2.5ul of each primer (10µM), 25ul Q5 Master Mix in a 50ul reaction).