Analysis of material Sample Clauses

Analysis of material. 3.2.10.1. Overall distribution of mGluR8-labeled elements from single immunoperoxidase-stained material. Five blocks of striatal tissue from 5 animals were cut out, mounted on resin blocks, and glued into place using cyanoacrylate glue. Ultrathin sections from the surface of these blocks were collected and scanned for the presence of immunoperoxidase- labeled structures that were easily distinguishable from unlabeled elements by their electron-dense reaction product. For each animal, 50 photographs of randomly selected immunoreactive elements were digitized at 20,000x. This resulted in a total surface of 7,200 µm2 of striatal tissue to be examined for each mGluR experiment. Immunoreactive elements were categorized as presynaptic (axons, terminals) or postsynaptic (dendrites, spines) neuronal structures on the basis of ultrastructural features. The relative abundance of each neuronal element for each receptor subtype in the striatum was calculated by dividing the number of labeled elements by the number of total labeled elements. To obtain the percent of labeled elements, the number of each type of labeled elements was divided by the number of total identified elements of that type. Significant differences were assessed by using a t-test (n=5). Data presented as average of percent labeled elements ± SEM.
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