Genotyping Method Sample Clauses

Genotyping Method. Genotyping will be accomplished by [***] specifically [***] the [***] for each [***]. All [***] from an [***] will be [***] with [***] and [***], and then [***] with [***]. A [***] will then be [***], the [***], and a [***]. The [***] a [***] to the [***] of the [***]. [***] subsequently [***] the [***] after [***] within the [***]. The [***] will be [***] by the [***] and [***] as [***]; each [***] a [***]. [***] of [***] will [***] a [***], while [***] of [***] will [***] a [***]. [***] will be [***] a [***] that is [***] as a [***]. A [***] and a [***] are also [***] with [***] of [***] as a [***] of [***].
Sample 1
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Genotyping Method. Blood samples were collected at the local institute and sent to the DNA bank at regular intervals for processing to allow analyses of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), protein and immortalised B cells. DNA purification and genotyping was performed by the Centre National de Génotypage in Paris. DNA was purified from whole blood samples (~10ml) preserved in BD Vacutainer EDTA tubes (Becton, Xxxxxxxxx and Company) using Gentra Puregene Blood Kit (Qiagen) according to the manufacturer’s instructions. Genotype information was collected at 582,982 markers using the Illumina HumanHap610 Genotyping BeadChip. Single nucleotide polymorphisms (SNPs) with call rates of < 98%, minor allele frequency < 1% or deviation from the Xxxxx-Xxxxxxxx equilibrium (p ≤ 1×10- 4) were excluded from the analyses. Individuals with an ambiguous sex code, excessive missing genotypes (failure rate > 2%), and outlying heterozygosity (heterozygosity rate 3 standard deviations from the mean) were also excluded. Identity-by-state similarity was used to estimate cryptic relatedness for individual using PLINK software. Closely related individuals with Identity-by-descent (IBD > 0.1875) were eliminated from the subsequent analysis. Population stratification for the GWAS data was examined by principal component analysis (PCA) using EIGENSTRAT software. The four HapMap populations were used as reference groups in the PCA and individuals with divergent ancestry (from CEU) were also excluded.
Sample 1

Related to Genotyping Method

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  • Service Providing Methodology 1.3.1 Party A and Party B agree that during the term of this Agreement, where necessary, Party B may enter into further service agreements with Party A or any other party designated by Party A, which shall provide the specific contents, manner, personnel, and fees for the specific services.

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  • Change in Accounting Method Neither Company nor any of its Subsidiaries has agreed to make, nor is it required to make, any material adjustment under Section 481(a) of the Code or any comparable provision of state, local, or foreign Tax Laws by reason of a change in accounting method or otherwise.

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