COLLECTION AND HANDLING. Specimen: Serum or plasma from samples collected in EDTA may be used. Do not use plasma collected in heparin. Fresh serum is the specimen of choice.
COLLECTION AND HANDLING. Specimen: The specimen may be serum, cerebrospinal fluid (CSF) or urine. Serum specimens should be free of hemolysis or lipemia. A fibrinogen band, which may obscure the beta-gamma zone, will appear in plasma samples. Storage: Fresh serum is the specimen of choice. If storage is necessary, samples may be stored covered at 2 to 8°C for 48 hours.
COLLECTION AND HANDLING. Specimen: Freshly collected serum and CSF are the specimens of choice. Both serum and CSF should be collected at the same time using conventional collection methods for clinical laboratory testing. Handling: CSF samples should be applied neat. Serum samples should be diluted 1:300 in 0.85% saline. Storage and Stability: Samples can be stored refrigerated at 2 to 8°C for up to 3 days or 2 weeks at -20°C.
COLLECTION AND HANDLING. Specimen: Serum is the specimen of choice. Plasma collected in heparin may be used. Anticoagulants containing oxalate, citrate or EDTA cannot be used because these substances inhibit the alkaline phosphatase activity.13 Patient Preparation: The patient should be fasting. Patients who have B or O blood group and are secretors may have an elevated ALP about two hours after a fatty meal.6, 12, 13, 18, 19
COLLECTION AND HANDLING. Use a 2.0 cc syringe to obtain the blood specimen as follows: Extracorporeal line: Using a two-syringe technique, flush the extracorporeal blood access line by withdrawing 2.0 cc of blood into a syringe and discarding it. Then use a second syringe to obtain a 0.5 cc sample for testing. In-dwelling line: Discontinue fluids drip, if necessary. Using a two-syringe technique, withdraw 2.0 cc of blood into a syringe and discard it. Then use a second syringe to obtain a 0.5 cc sample for testing. Venipuncture: Using a two-syringe technique, withdraw 2.0 cc of blood into a syringe and discard it. Then use a second syringe to obtain a 0.5 cc sample for testing.
COLLECTION AND HANDLING. Specimen: Plasma from whole blood collected in sodium citrate as an anticoagulant. Specimen Preparation: Collect the blood specimen in either 3.2% (0.109 M) or 3.8% (0.129 M) sodium citrate. Add nine parts whole blood to one part sodium citrate solution. Centrifuge the blood sample immediately after collection at 1000 x G for 10 minutes. Store the plasma at 2 to 8°C until testing is performed. Plasma stored at 2 to 8°C must be tested within four hours after sample collection. Plasma is stable at -20°C for one month. Plastic tubes must be used for storage and testing.
COLLECTION AND HANDLING. Specimen: For serum protein analysis, freshly collected serum is the specimen of choice as plasma samples will contain a large fibrinogen peak between the beta and gamma fractions. Storage and Stability: Samples can be stored at 2 to 8°C for up to 7 days and up to 6 months at -20°C. If samples are to be stored frozen, they should be refrigerated immediately and frozen within 8 hours of collection. Storing samples at 2 to 8°C can result in protein degradation, particularly, but not exclusively of complement fractions. Consequently, after 7 days storage at 2 to 8°C, detection of a distinct beta2 region may no longer be possible. DO NOT store samples at room temperature - the sample will degrade rapidly. Samples which contain cryoglobulins may become viscous or turbid after refrigeration or freezing. It is advisable to warm these samples to room temperature before analysis. PROCEDURE Materials provided: The following materials are provided: Sample Test Size Cat. No. 50 1803C Anti-human IgG antisera 1 vial Anti-human IgA antisera 1 vial Anti-human IgM antisera 1 vial Anti-human kappa antisera 1 vial Anti-human lambda antisera 1 vial Materials provided but not contained in the above kit: Item Cat. No. V8 Velocity Analyzer 1800 V8 Nexus CE Analyzer 1825 V8 Storage Buffer 1831 V8 Maintenance Buffer 1832 V8 Serum Protein SPE Kit 1805 V8 Clinical Waste Drawer Inserts 1820 Materials required but not provided: Item Uncapped primary tubes These instructions are for standard mode versus touch screen. (For correct installation of all consumables, please refer to the Operator Manual)
1. Ensure that the waste container drawer is on-board.
2. Before switching on the V8, ensure that the Storage Buffer, Maintenance Buffer and disposable cups are on-board and in their correct positions.
3. Launch Platinum and begin a new V8 session. In Platinum, select "V8 SYSTEM" from the drop down menu, click "SELECT DEFAULT METHOD" and ensure the relevant Immunodisplacement assay is selected. For reflex testing or individual test ordering, please refer to the Operator Manual.
4. Switch on the V8 as instructed in the Operator Manual.
5. To conduct Immunodisplacement testing, install the relevant Serum Protein Buffer and Diluent if required.
6. Ensure that the Immunodisplacement reagent barcode information is loaded into Platinum by selecting "V8 SYSTEM" from the drop down menu. Click "DEFINE REAGENTS" and ensure the barcode information for each antisera type has been entered and that the lo...
COLLECTION AND HANDLING. Specimen: Serum is the specimen of choice. Plasma from blood specimens collected in heparin or EDTA may be used. Anticoagulants containing oxalate should not be used due to the inhibition of LD by oxalate11. Plasma samples should be well centrifuged to eliminate platelets which contain LD12.
1. Hemolysis: Erythrocytes contain 100-150 times more LD than does serum. Hemolysis may contribute to error in assessment of LD1,2 acti-vity1-2,11.
2. Uremic sera: LD activity is reduced in uremic sera due to the presence of the inhibitors, urea and oxalate, and other unidentified substances. Urea affects LD5 more than LD1 13.
3. Acetone and chloroform inactivate all isoenzymes of LD except LD1 14.
4. For the effect of various drugs on LD activity, refer to Young, et al15. Storage and Stability: Serum should be tested as soon as possible after collection. Fresh serum is the specimen of choice because different storage conditions have varying effects on the isoenzymes11,14,16,17. No one storage temperature is optimum for all the isoenzymes. When storage is required, serum samples may be stored at 15 to 30°C or at 2 to 6°C for up to 48 hours. Storage at 2 to 6°C permits simultaneous storage of serum for both CK and LD isoenzyme studies11. Do not freeze the sample as LD5 is very unstable at freezing temperatures11. Materials Provided: The following materials are provided in the SPIFE LD Isoenzyme Kits. Individual items are not available separately. 60 sample 3335 40 sample 3336 20 sample 3337 SPIFE LD Isoenzyme Gels (10) LD Isoenzyme Reagent (20 x 1.0 mL) LD Isoenzyme Diluent (2 x 10 mL) REP Blotter C (10) Blade Applicator Kit - 20 Sample Citric Acid Destain (1 pkg) SPIFE Touch 1068 QuickScan Touch 1690 Quick Scan 2000 1660 CK/LD Control 5134 REP Prep 3100 Gel Block Remover 1115 SPIFE Reagent Spreaders 3706 SPIFE 20-100 Dispo Cup Tray 3366 SPIFE Dispo Sample Cups (Deep Well) 3360 Chamber Cover 8JP34012
COLLECTION AND HANDLING. Throughout the procedure for determination of activated partial thromboplastin times (aPTT) all test tubes, syringes and pipettes must be plastic or siliconized glass. Specimen: Plasma obtained from whole blood collected with sodium citrate as an anticoagulant is the specimen of choice. The concentration of the sodium citrate should be 3.8% (0.129 M) or 3.2% (0.109 M).
COLLECTION AND HANDLING. Specimen: Serum is the specimen of choice. Plasma from blood specimens collected in heparin or EDTA may be used. Anticoagulants containing oxalate should not be used due to the inhibition of LD by oxalate.11 Plasma samples should be well centrifuged to eliminate platelets which contain LD.12
