Feeding. The organisms shall not be fed during testing. During culturing, holding, and acclimation, a sufficient quantity of deciduous leaves, such as maple, aspen, or birch, should be placed in the culture and holding containers to cover the bottom with several lay- ers. These leaves should be aged for at least 30 days in a flow-through system before putting them in aquaria. As these leaves are eaten, more aged leaves should be added. Pelleted fish food may also be added. (2) Facilities—(i) Apparatus—(A) Fa- cilities needed to perform this test in- clude: (1) Containers for culturing, acclimating and testing gammarids; (2) Containers for aging leaves under flow-through conditions; (3) A mechanism for controlling and maintaining the water temperature during the culturing, acclimation and test periods; (4) Apparatus for straining particu- late matter, removing gas bubbles, or aerating the dilution water, as nec- xxxxxx; and (5) An apparatus for providing a 16- hour light and 8-hour dark photoperiod with a 15- to 30-minute transition pe- riod.
Appears in 9 contracts
Samples: Testing Consent Agreements and Test Rules, Testing Consent Agreements and Test Rules, Testing Consent Agreements and Test Rules
