Microscopic Examinations Sample Clauses

Microscopic Examinations. ⏺ Wet mount: The saline wet prep is easily prepared and is used for the rapid detection of Trichomonas vaginalis, and “clue” cells associated with bacterial vaginosis. 🔾 Materials Required: Microscope, slides, cover slips (22 x 22 mm), saline, cotton-tipped swabs, bright ?eld microscope. �� Preparation of Wet Mount Slide: ⏵ Place a drop of saline on a glass slide; then mix with a drop of vaginal fluid collected on swab. ⏵ Cover a wet preparation with a coverslip and examine microscopically at x 100 magnification prior to examination at x400 magnification. 🔾 Interpretation: Trichomonads are best recognized by their typical jerky motility. Trichomonas vaginalis is a pear shaped parasite. It contains a central nucleus, four anterior flagella and an undulating membrane. Clue cells are squamous epithelial covered with many small coccobacillary organisms, giving a 66 STI SOP - Sep 07 stippled,granular aspect ; the edges of these cells are not clearly defined, owing to large number of bacteria present and the apparent disintegration of the cells. In most patients with BV, a mixture of normal exfoliated vaginal epithelial cells and 20% or more clue cells will be seen. ⏺ KOH Mount: The KOH preparation is used to detect yeast. 🔾 Materials Required: Microscope, slides, coverslips (22 x 22 mm), 10% KOH, cotton-tipped swabs, bright?eld microscope. 🔾 Preparation of KOH Mount Slide: ⏵ Collect the vaginal specimen on a swab, and then roll the swab on a small area of the slide. ⏵ Add a drop of 10% KOH (potassium hydroxide) and mix with a wooden applicator or swab. ⏵ Sniff for a “?shy” odor (Whiff test). ⏵ Cover with a coverslip; avoid trapping air bubbles. ⏵ Examine the slide microscopically. Scan for pseudohyphae on low power. Confirm the presence of pseudohyphae and locate yeast buds on high dry. 🔾 Interpretation: Yeasts are round to ovoid cells, approximately 4 m in diameter, showing typical budding. Yeast cells may appear in chains known as pseudohyphae. ⏺ Gram Staining: The Gram stain is useful in the diagnosis of gonorrhea. Both the numbers of polymorphonuclear leukocytes (PMNLs) and microbial ?ora present can be assessed. 🔾 Materials required: Cotton-tipped swabs, microscope, slides, methanol, Gram stain reagents: crystal violet, Gram's iodine, decolorizer (50:50 mixture of acetone and 95% ethanol), safranin, sink or staining tray with water source, paper towels or blotting paper, immersion oil. 🔾 Preparation of Smear: ⏵ Carefully roll the swab (urethral or c...
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Related to Microscopic Examinations

  • Eye Examinations For all covered EMPLOYEES required to use VDTs on average at least two (2) hours per day, the Department will provide a base line eye examination at the Occupational Safety and Health facility ("OSH"), followed by an eye examination at OSH once a year.

  • Tuberculosis Examination The examination shall consist of an approved intradermal tuberculosis test, which, if positive, shall be followed by an X-ray of the lungs. Nothing in Sections 5163 to 5163.2, inclusive, shall prevent the governing body of any city or county, upon recommendation of the local health officer, from establishing a rule requiring a more extensive or more frequent examination than required by Section 5163 and this section. § 5163.2. Technician taking X-ray film; Interpretation of X-ray The X-ray film may be taken by a competent and qualified X-ray technician if the X-ray film is subsequently interpreted by a licensed physician and surgeon.

  • Promotional Examinations All promotional examinations shall be competitive and shall be conducted in the same manner as open examinations except that admission to a promotional examination shall be restricted to persons in the employ of the County who have served at least six months following regular appointment to the classified service and who meet the published requirements for the examination. The Commission shall determine whether an examination shall be held on a promotional or open basis. 1492 Disposition of Papers and Records Prescribed application forms of persons who fail to appear for the written test and examination records of candidates who fail to qualify in the written test shall be destroyed at any time after thirty days from the date of promulgation of the eligible list. Examination records of candidates who qualify in an examination shall be retained during the life of the eligible list or for 15 months, whichever is longer, and the examination records of each appointee shall be filed in his/her permanent personnel folder. Following the period during which competitors may inspect their examination papers, and after their ratings in each part of the examination have been transferred to examination records, examination materials such as question booklets, answer sheets and work papers may be destroyed, but general qualification appraisal sheets and applications shall be retained at least 15 months from the date of the promulgation of the eligible list. APPOINTMENT

  • Medical Examinations An employee may be required by the Employer, at the request of and at the expense of the Employer, to take a medical examination by a physician of the employee's choice. Employees may be required to take skin tests, x-ray examination, vaccination, inoculation and other immunization (with the exception of a rubella vaccination when the employee is of the opinion that a pregnancy is possible), unless the employee's physician has advised in writing that such a procedure may have an adverse affect on the employee's health.

  • Physical Examinations Where the Employer requires an employee to take a physical examination, doctor's fees for such examination shall be paid by the Employer. Except prior to commencement of employment and the first four (4) weeks of employment, such examinations shall be taken during the employee's working hours without loss of pay to the employee.

  • Health Examinations The Employer shall provide at no cost to the employee, such medical tests, health examinations and surveillance/monitoring as may be required as a condition of employment and/or as a result of regulated hazards encountered after employment.

  • Polygraph Examinations No employee shall be compelled to submit to a polygraph examination against his/her will. No disciplinary action or other recrimination shall be taken against an employee refusing to submit to a polygraph examination, nor shall any comment be entered anywhere in the investigator’s notes or anywhere else that the employee refused to take a polygraph examination, nor shall any testimony or evidence be admissible at a subsequent hearing, trial, or proceeding, judicial or administrative, to the effect that the employee refused to take a polygraph examination.

  • Medical Examination Where the Employer requires an employee to submit to a medical examination or medical interview, it shall be at the Employer's expense and on the Employer's time.

  • Annual Physical Examination A permanent employee shall be granted up to one day per year with pay for the purpose of a comprehensive physical examination provided that the verification of such an examination is submitted to the District.

  • Polygraph Examination No employee shall be compelled to submit to a polygraph examination. No disciplinary action whatsoever shall be taken against an employee refusing to submit to a polygraph examination; nor shall any comment be anywhere recorded indicating that an employee offered to take, took or refused to take a polygraph examination unless otherwise agreed to in writing by the parties; nor shall any testimony or evidence of any kind regarding an employee's offer to take, refusal to take, or the results of a polygraph examination be admissible in any proceeding pursuant to this Agreement, unless otherwise agreed to in writing by the parties.

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