Quantitation of DNA samples Sample Clauses

Quantitation of DNA samples. The quality of the DNA samples was checked by NanoDrop (DNA quality analyses). NanoDrop accurately measures dsDNA samples up to 3700 ng/µl without dilution. It automatically detects the high concentration and utilizes the 0.2mm path length to calculate the absorbance as shown in Table 2.1. For NanoDrop spectrophotometry, 1.0 µl of sample was used to quantify DNA concentration. A blank of nuclease-free water, was used to optimise the instrument. Sample pedestals were first cleaned with nuclease- free water and ethanol prior to use. Table 2.1 Sample of DNA quality analyser 260/280: the ratio of absorbance at 260 and 280 nm is used to assess the purity of DNA. A ratio of 1.8 is accepted as pure for DNA. If the ratio is lower, it may indicate the presence of contaminants such as protein or phenol that absorb strongly at or near to 280 nm. After checking the quality of the extracted DNA, samples were diluted to 10 ng/µl. The high quality, intact genomic DNA from the buffy coat fraction of the RISCK subjects was frozen and stored in 5 x 94 xxxxx and 1 x 60 on 96 well DNA plates, and 3 x 94 xxxxx and 1 x 55 xxxxx on 96 well DNA plates for MARINA subjects. Samples were defrosted as needed for analysis.
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