Thawing of cryopreserved cells Sample Clauses

Thawing of cryopreserved cells. Vials were taken from -80⁰C freezer or liquid nitrogen and transferred to a 37⁰C water bath until defrosted. Cells were then taken into 5-10mls of appropriate complete media+10% FCS in a 15ml falcon tube to dilute out DMSO, and centrifuged at 200xg for 5 mins to pellet cells. Cells were then resuspended in complete media and transferred to culture vessels. Cells were cultured for at least 48h before being used in assays.
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