Transformation of Competent Bacteria Sample Clauses

Transformation of Competent Bacteria. Bacteria were thawed on ice and 20μl per condition was added to pre-chilled tubes. 1-100ng of DNA was added and incubated on ice for 30 mins. Tubes were heat shocked for 1 minute at 37⁰C and returned to ice for a further 5 mins. 200μl of SOC media was added and bacteria were incubated with agitation at 37⁰C for 1 hour before spreading onto agar plates.
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