STEP METHOD Sample Clauses

STEP METHOD. NOTE: If the staining chamber was last used to stain a gel, the SPIFE Touch software has an automatic wash cycle prompted by the initiation of the SPIFE Touch CK test. To verify the status of the stainer chamber, use the arrows under the STAINER UNIT to select the appropriate test, place the empty Gel Holder into the stainer chamber and press START. If washing of the staining chamber is necessary, the prompt “Vat must be washed. Remove gel and install gel holder.” will appear. Press RETRY to begin the stainer wash. The cleaning process will complete automatically in about 7 minutes. To avoid delays after incubation, this wash cycle should be initiated at least 7 minutes prior to the end of the run .
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STEP METHOD. Chamber Preparation
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STEP METHOD. I. Instrument Parameters
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STEP METHOD. NOTE: If a SPIFE procedure requiring a stain has been run prior to running the LD gels, the stainer unit must be cleaned/washed before washing the gel. The new software version 1.20 has an automatic wash cycle prompted by initiation of a test which does not use the stainer unit for staining when the previous test did use the stainer for staining. To avoid delays after elec- trophoresis, this wash cycle should be initiated at least seven (7) minutes prior to the end of the run. To verify the status, press the TEST SELECT/ CONTINUE button on the stainer until the appropriate test is selected. Place an empty Gel Holder in the stainer unit. If cleaning is required, the “Wash 1” prompt will appear, followed by “Plate out, Holder in” prompts. Press “Continue” to begin the stainer wash. The cleaning process will complete automatically in about 7 minutes. The unit is then ready to process the gel after incubation. Reagent Preparation Reconstitute two vials of the LD Isoenzyme Reagent with 1.0 mL LD Isoenzyme Diluent each. Mix well by inversion. Sample Preparation If testing 41-60 samples, remove three Applicator Blades from the packag- ing. If testing fewer samples, remove the appropriate number of Applicator Blades from the packaging. Place the three Applicator Blades into the vertical slots in the Applicator Assembly identified as 2, 9 and 16. If using fewer Applicator Blades, place them into any of the three slots noted above. Load Sample 1 00:02 20°C SPD6 No Prompt Load Sample 2 00:02 20°C SPD6 No Prompt Load Sample 3 00:02 20°C SPD6 No Prompt Load Sample 4 00:30 20°C SPD6 No Prompt Apply Sample 1 00:30 20°C SPD6 LOC1 No Prompt Electrophoresis 1 6:00 10°C 600 Volt 100mA No Prompt Apply Reagent 1 45°C 4 cycles To Continue, (Continue) Incubate 1 20:00 45°C No Prompt END OF TEST No Prompt Destain 1 15:00 REC=REV VALVE=2 No Prompt Wash 1 10:00 REC=REV VALVE=7 No Prompt Dry 1 25:00 70°C No Prompt END OF TEST Electrophoresis Open the chamber lid. Place the Cup Tray with samples on the SPIFE 3000. Align the holes in the tray with the pins on the instrument. Place a reconstituted vial of reagent into each outer hole of the reagent bar, ensuring that the vials are pushed down as far as they can go. Close the chamber lid. With LD on the display, press the START/STOP button. An option to either begin the test or skip the operation will be presented. Press START/STOP to begin. The SPIFE 3000 will apply the samples, electrophorese, spread reagent and beep. Open the chamber...
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STEP METHOD. I. Calibration
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Related to STEP METHOD

  • Allocation Method (Choose one of a. or b.): a. [ ] All the same. Using the same allocation method as applies to the Signatory Employer under this Election 28. b. [ ] At least one different. Under the following allocation method(s): .

  • Measurement method An isolation resistance test instrument is connected between the live parts and the electrical chassis. The isolation resistance is subsequently measured by applying a DC voltage at least half of the working voltage of the high voltage bus. If the system has several voltage ranges (e.g. because of boost converter) in conductively connected circuit and some of the components cannot withstand the working voltage of the entire circuit, the isolation resistance between those components and the electrical chassis can be measured separately by applying at least half of their own working voltage with those components disconnected.

  • Billing Method 2.6.1 To receive payment for services rendered pursuant to this contract the Contractor shall submit a fully completed invoice for work previously performed to: 2.6.2 At a minimum, the invoice shall detail the following information: 2.6.2.1 Unique invoice number; 2.6.2.2 Contractor’s name, address, and telephone number; 2.6.2.3 Date of invoice and/or billing period; 2.6.2.4 Applicable Contract No.;

  • Payment Methodology The Contractor shall be compensated based on the Service Rates in Attachment for units of service authorized by the Institution in a total amount not to exceed the Contract Maximum Liability established in Section C. 1. The Contractor’s compensation shall be contingent upon the satisfactory completion of units of service or project milestones identified in Attachment B. The Contractor shall submit invoices, in form and substance acceptable to the Institution with all of the necessary supporting documentation, prior to any payment. Such invoices shall be submitted for completed units of service or project milestones for the amount stipulated.

  • Balance Computation Method For all dividend-bearing Accounts, dividends are calculated by the average daily balance method which applies a daily periodic rate to the average daily balance for the average daily balance calculation period. The average daily balance is determined by adding the full amount of the principal in Your Account for each day of the period and dividing that figure by the number of days in the period. Accrual on Noncash Deposits. For dividend-bearing Accounts, dividends will begin to accrue on the business day that You deposit noncash items (e.g. checks) into Your Account.

  • Change in Accounting Method Neither Company nor any of its Subsidiaries has agreed to make, nor is it required to make, any material adjustment under Section 481(a) of the Code or any comparable provision of state, local, or foreign Tax Laws by reason of a change in accounting method or otherwise.

  • Accounting Method For both financial and tax reporting purposes, the books and records of the Company shall be kept on the accrual method of accounting applied in a consistent manner and shall reflect all Company transactions and be appropriate and adequate for the Company’s business.

  • Settlement Method For any Option, Net Share Settlement; provided that if the Relevant Settlement Method set forth below for such Option is not Net Share Settlement, then the Settlement Method for such Option shall be such Relevant Settlement Method, but only if Counterparty shall have notified Dealer of the Relevant Settlement Method in the Notice of Final Settlement Method for such Option.

  • Voting Method Unless otherwise provided by law, when a vote is taken upon any measure before the Board, with a quorum being present, a majority of the votes cast shall determine its outcome. A vote of "abstain" or "present," or a vote other than "yea" or "nay," or a failure to vote, is counted for the purposes of determining whether a quorum is present. A vote of "abstain" or "present," or a vote other than "yea" or "nay," or a failure to vote, however, is not counted in determining whether a measure has been passed by the Board, unless otherwise stated in law. The sequence for casting votes is rotated. On all questions involving the expenditure of money and on all questions involving the closing of a meeting to the public, a roll call vote shall be taken and entered in the Board's minutes. An individual Board member may request that a roll call vote be taken on any other matter; the President or other presiding officer may approve or deny the request but a denial is subject to being overturned by a majority vote of the members present. Any Board member may request that his or her vote be changed before the President announces the result. Any Board member may include a written explanation of his or her vote in the District file containing individual Board member statements; the explanation will not be part of the minutes. The Board Secretary shall keep written minutes of all Board meetings (whether open or closed), which shall be signed by the President and the Secretary. The minutes include:

  • Payment Method Payment shall be made by the Contractor to the Subcontractor as follows: (choose one) ☐ - Immediately upon completion of the Services to the satisfaction of the Contractor. ☐ - Within ____ business days after completion of the Services to the satisfaction of the Contractor. ☐ - Shall be paid on a ☐ weekly ☐ monthly ☐ quarterly ☐ other ______________________ basis. If the Subcontractor completes the Services to the satisfaction of the Contractor, before the full amount or balance has been fully paid, any remaining amount shall be payable immediately. ☐ - Other: ________________________________________________________

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