Backgrounds and Aims Sample Clauses

Backgrounds and Aims. Task 2 aims to provide a clear-cut evidence for BFT population structure fulfilling the gap represented by the microsatellite genotyping of reference samples in the Mediterranean at an extensive spatial scale and across a short-term temporal scale to check interannual stability of genetic structure. In fact, previous microsatellite analyses on BFT (Xxxxxxxx et al. 2004, 2006, 2007, Xxxxxxxx et al. 2010, 2013) have never been conducted on experimental designs accomplishing simultaneously the use of reference samples (Larvae or Young-of-the-Year) collected during the same spawning season (from June to September) of multiple consecutive years in the same Mediterranean spawning subareas. In addition, the Task 2 aims to overwhelm the methodological constraints represented by the use of a limited number of microsatellite loci (< 20) in the previous analyses and by the use of panels of microsatellites largely different among studies carried out including both the Mediterranean and Gulf of Mexico samples that prevent any fully reliable comparative issue. Thus, a pan-Atlantic study based on a large panel of microsatellite loci (> 20) used to genotype reference samples from both ascertained spawning areas is considered necessary to assess genetic differences within and between each area and provide clear-cut evidence for population structure both in the Mediterranean and in the Gulf of Mexico. To accomplish such aims a Research Collaborative Agreement between scientists of UNIBO- COMBIOMA (GBYP Consortium Partners) and of Stanford University – Marine Xxxxxxx Station (B. Block and X. Xxxx) has been formally activated to exchange genetic resources (primer sequences and PCR conditions for 18 microsatellite loci newly developed by Stanford University researchers and already used to type Gulf of Mexico reference samples and 10 reference gDNAs from Gulf of Mexico for genotype intercalibration) and merge genetic data (Mediterranean and Gulf of Mexico intercalibrated genotypes) to achieve a pan-Atlantic BFT population structure pattern.
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Related to Backgrounds and Aims

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  • Project Background 6.1.1. Brief description of Contracting Agency’s project background and/or situation leading to this Project

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