Expression. Employee demonstrates the ability to convey ideas and suggestions orally and in writing in a manner appropriate to the situation and his/her position. Exceeds Meets Occasional Problem(s) Consistent Problem(s) Comments:
Expression. It includes preliminary and final products and any materials and information developed for the purposes of producing those final products. "Works" does not include articles submitted to peer review or reference journals or independent research projects.
Expression. An open-array constructor consists of one or more expressions separated by commas and enclosed in square brackets. Each expression must be assignment compatible with the element type of the open-array parameter. The use of an open- array constructor corresponds to creating a temporary array variable, and initializing the elements of the temporary array with the values given by the list of expressions. For example, given the declaration of the Sum function above, the statement X := Sum([A, 3.14159, B + C]); corresponds to Temp[0] := A; Temp[1] := 3.14159; Temp[2] := B + C; X := Sum(Temp); where Temp is a temporary array variable with three elements of type Double. Type variant open-array parameters A type variant open-array parameter allows an open array of expressions of varying types to be passed to a procedure or function. A type variant open-array parameter is declared using the syntax array of const The array of const syntax is analogous to array of TVarRec. The TVarRec type is a variant record type which can represent values of integer, boolean, character, real, string, pointer, class, and class reference types. The TVarRec type is declared in the System unit as follows type TVarRec = record case VType: Byte of vtInteger: (VInteger: Longint); vtBoolean: (VBoolean: Boolean); vtChar: (VChar: Char); vtExtended: (VExtended: PExtended); vtString: (VString: PString); vtPointer: (VPointer: Pointer); vtPChar: (VPChar: PChar); vtObject: (VObject: TObject); vtClass: (VClass: TClass); end; The VType field determines which value field is currently defined. Notice that for real or string values, the VExtended or VString field contains a pointer to the value rather than the value itself. The vtXXXX value type constants are also declared in the System unit: const vtInteger = 0; vtBoolean = 1; vtChar = 2; vtExtended = 3; vtString = 4; vtPointer = 5; vtPChar = 6; vtObject = 7; vtClass = 8; The MakeStr function below takes a type variant open-array parameter and returns a string that is the concatenation of the string representations of the arguments. The AppendStr, IntToStr, FloatToStr, and StrPas functions used by MakeStr are defined in the SysUtils unit. function MakeStr(const Args: array of const): string; const BoolChars: array[Boolean] of Char = ('F', 'T'); var
Expression. Thus, we employed a mutagenesis analysis to determine whether the inhibitory effect of miR- 324-5p is mediated directly by the putative target region in the 3’UTR of Kv4.2. Accordingly, we designed a reporter construct, ffluc-Kv4.2-3’UTR-MUT, bearing a two nucleotide-mutation in the putative target region of 3’UTR of Kv4.2 (Fig. 3-2A). This point mutation would disrupt the predicted base pairing between miR-324-5p and 3’UTR of Kv4.2. Therefore, the inhibitory effect of miR-324-5p should be eliminated. Indeed, overexpression of miR-324-5p had no significant effect on the luciferase activity of ffluc- Kv4.2-3’UTR-MUT (Fig. 3-2B), suggesting that the target region in 3’UTR is a cis- acting inhibitory element. In line with this observation, ffluc-Kv4.2-3’UTR-MUT exhibits higher luciferase activity than ffluc-Kv4.2-3’UTR (33.0%±8.6%), suggesting that the mutated Kv4.2 construct cannot be regulated by endogenous miR-324-5p (Fig. 3- 2C). Overall, these data suggest that the putative target region of xxXXX-324-5p within the Kv4.2 3’UTR is responsible for the inhibitory function of miR-324-5p on the Kv4.2 reporter constructs, corroborating our hypothesis that miR324-5p directly affects Kv4.2 expression. miR-324-5p is localized to neurons Kv4.2 is highly expressed in the brain and is the predominant potassium channel that composes A-type currents in the hippocampus (Xxxx et al., 2006). Given the potential function of miR-324-5p for Kv4.2 expression, we hypothesized that miR-324- 5p might regulate Kv4.2 in vivo in the brain. We therefore assessed the expression and localization of miR-324-5p in neurons. Fluorescence in situ hybridization (FISH) using digoxigenin labeled LNATM probes was performed to investigate the neuronal localization of miR-324-5p in both brain tissues and cultured hippocampal neurons. miR- 324-5p is localized to both cell bodies and dendrites in the hippocampus of the brain (Fig. 3-3A-C). Correspondingly, miR-324-5p shows expression in 14 day in vitro (DIV) cultured hippocampal neurons (Fig. 3-3D). Overall, these data demonstrate that miR- 324-5p is expressed in neurons and support the hypothesis that miR-324-5p regulates Kv4.2 expression in vivo in the brain. miR-324-5p regulates endogenous Kv4.2 protein levels in primary neurons To investigate whether miR-324-5p modulates the expression of endogenous Kv4.2 protein, we overexpressed or inhibited miR-324-5p in high-density cultured neurons. Lentiviruses expressing both GFP and miR-324-5p or a scr...
Expression. For each image, we calculated how many participants chose the targeted emotion. Overall the agreement between chosen and targeted emotions was 82% (median 88%, SD 19%). Average choice rates per targeted emo- tion are depicted in Figure 4. An ANOVA of the arcsine-transformed agreement rates (Xxxxx, 1971) revealed a significant effect of expression, F(7, 1080)=168.2, pB.01, h2p=.52. Post hoc tests showed that agreement was significantly higher for happiness (mean 98%, SD=3%), and significantly lower for contempt (mean 50%, SD=15%), compared to all other expressions (means between 80 and 90%). Table 2. Intraclass correlations for all rating dimensions (but the emotion rating) Dimension ICC(1, 1) ICC(1, k) Dutch adultAttractiveness .31 .99 Intensity .20 .83 Clarity .19 .83 Genuineness .13 .75 Valence .44 .94 Dutch childrenAttractiveness .24 .94 Intensity .26 .88 Clarity .22 .85 Genuineness .09 .67 Valence .48 .95 A close look at Figure 4 reveals that, for some expressions, off-diagonal responses were not equally distributed across chosen expressions: Faces with intended surprise were sometimes confused with fear (7%), and, vice versa: intended fear was sometimes confused with surprise (8%); intended disgust was sometimes mistaken for either anger (7%) or contempt (8%); and for intended contempt participants responded fre- quently either other (24%) or neutral (12%). As the above-reported agreement rates do not take response bias into account, we additionally determined and analysed unbiased hit rates (Xxxxxx, 1993). Unbiased hit rates can vary between 0 and 1, where a hit rate of 1 indicates not only that a stimulus category (e.g., happy faces) was always identified correctly (e.g., that happy faces were always categorised as happy), but additionally that the corresponding response (e.g., response ‘‘happy’’) was always used correctly (e.g., that the response ‘‘happy’’ was only given for happy faces). Lower unbiased hit rates result if either stimuli from a category are not classified correctly (e.g., happy faces categorised as angry) or if the corresponding response was also used for stimuli from other categories (e.g., that the response ‘‘happy’’ was also given to angry or surprised faces). Unbiased hit rates were com- puted as follows: Per participant and for each gaze direction, we first created a choice matrix with targeted and chosen expressions as rows and columns, respectively. Next, the number of ratings in each cell was squared and divided by the prod...
Expression. The expression of a luciferase reporter containing 3’UTR of Kv4.2 could be influenced by overexpression or inhibition of miR-324-5p (Fig. 3-1). Furthermore, miR-324-5p regulates endogenous Kv4.2 protein expression (Fig. 3-4A and
Expression. Our results suggest that miR-324-5p may regulate Kv4.2 expression and functions during neuronal development and plasticity. My thesis work provides new insights into the mechanisms regulating neuronal excitability. Dysregulation of these mechanisms might be involved in epileptogenesis, e.g. in FXS. Our findings therefore provide a molecular basis for future studies toward a better understanding the pathogenesis of FXS and other forms of epilepsy.
Expression. Salonetwork wants to give you the opportunity to express yourself and communicate with people within the same industry. This platform allows you to ‘Tell the Network’ your ideas or interests and upload photos and videos to support your comments. We also want to help you be fully informed of new product and services, which are offered by businesses within your industry. These will be show via ‘Tell the Network’ or via our ads, which are paid for via these businesses. Any content or information which you upload to Salonetwork can be see by all members who are registered. You acknowledge the risk that you or your business may be mistakenly associated with content about others when members of Salonetwork know you or your business were mentioned in the news, At Salonetwork we want you to share your ideas and interests while ensuring you feel you are in a safe and secure environment. We will always ensure to use reasonable care when providing our services to you, however we cannot guarantee that all of our services will function without any disruptions. Therefore Salonetwork will not tolerate misuse of the site, products or misconduct towards any other member. If we learn that this is the case Salonetwork will take appropriate action for example, removing content, disabling accounts, blocking accounts or contacting the law. All members have the option to report any post to the Salonetwork team if they feel a post is inappropriate or offensive in anyway. By using the Salonetwork service you may encounter content or information, which may be inappropriate, incomplete, misleading, offensive, illegal, or otherwise harmful. Salonetwork cannot take any responsibility and you agree we are not responsible for others (including other members) content or information which is uploaded to Salonetwork. We cannot always ensure misuse of the platform and you agree we are not responsible for any misuse, however the Salonetwork team will do their upmost to ensure inappropriate content is removed or any posts reported will be investigated with immediate effect. Termination: Salonetwork reserves the right to suspend, restrict or terminate your account at any time. Salonetwork also have the right to limit your use of the platform. If you decide to terminate your contact the following will survive: • We can use and disclose your feedback • Members and/or Visitors rights to further re-share content and information you shared through the Service to the extent copied or re-shared prior...
Expression. The terms and expressions referred to in this agreement shall have the same meaning with those used in the Loan Agreement.
