Technical Plan. A. Sorghum Breeding 20
B. Sorghum Composition/Conversion Testing 22
C. Genotyping/Marker-Assisted-Breeding 23 D. Technology Development 24 E. Sorghum Genome Platform 26 F. Bioenergy Traits/Gene Discovery 27
I. Executive Summary:
Technical Plan. [***] DNA marker development: (budgeted in MAB platform development) — sequence genes flanking [***] for [***] discovery — sequence the [***] corresponding to [***] — sequence genes from [***], [***] germplasm used in breeding — optimize Taqman assays for MAB — assay [***] plants for [***]
Technical Plan. 2.1 Optimize [***] profiling technology on the [***].
Technical Plan. Phase I alignment of DOE [***] to [***] (20[***]).
Technical Plan. The Offeror’s proposal shall describe its understanding of the requirements of the SOW and specifically address how the work would be accomplished as follows:
Technical Plan. The Offeror’s proposal shall demonstrate its understanding of the requirements of the task order and specifically address how the work would be accomplished as follows:
Technical Plan. How well does the offeror’s proposal demonstrate an understanding of the problem, comply with the objectives of the program stated in the ORS Architecture Guidebook, and offer a sound approach?
Technical Plan. Optimize SAGE digital expression profiling technology on the Solexa sequencer. 2.2 Map [***] to genes and genome locations. 2.3 Collect reference profiles of selected treatments/tissues ([***], [***], [***]).
Technical Plan. Phase I alignment of DOE sequence assemblies to [***] (20[***]).
Technical Plan. Map QTL for [***] and [***] traits in [***]/[***] populations: — phase I mapping population; cross [***] lines Xx. Xxxxxx identified in 20[***] — phase II mapping population; cross [***] X [***]-line — select parents based on [***] and [***] data — assay [***] among top [***] lines (in progress) — assay diversity of [***] (CERES)/ [***] among [***] lines — cross [***] lines (in progress), [***], [***]; assay [***] lines (20[***]) — select for [***] lines that have fairly [***] ([***])/ [***] for [***] — population size of ~[***] is sufficient for initial QTL mapping — assay [***] ([***], etc) and [***] at end of season, replicated — College Station; other sites when time, funding permit — construct a [***] for QTL mapping (use RSL/Solexa technology) — conduct gene expression assays, [***] (Solexa/qRT-PCR) — expand sub-populations for [***] — identify [***]/[***]/[***] region, [***], generate a large [***] population — identify useful [***], phenotype for target trait(s) — candidate [***], [***] analysis, and validation — capture IP — [***] germplasm for [***] — translate to other [***]
