METHODS. 3.2.1. Reagent setup • Mitochondria Isolation Buffer (MIB): 75 mM sucrose, 225 mM d-mannitol, 1 mM EGTA, 1 mM EDTA, 5 mM HEPES, pH 7.40 in ultra-pure water. The pH was adjusted to 7.4 with NaOH. The solution was then filtered and stored at - 20°C for up to 3 months. • Optipreptm Diluent (OD): 250mM sucrose, 2mM EGTA, 2mM EDTA, 35mM Hepes, pH 7.4 in ultra-pure water. The pH was adjusted to 7.4 with NaOH. The solution was then filtered and stored at -20°C for up to 3 months. • Optipreptm Diluent 2 (OD2): 75 mM sucrose, 225 mM d-mannitol, 1 mM EGTA, 1 mM EDTA, 20 mM HEPES, pH 7.40 in ultra-pure water. The pH was adjusted to 7.4 with NaOH. The solution was then filtered and stored at - 20°C for up to 3 months. • 20% (vol/vol) Optipreptm solution: 2 ml of OD2 were mixed with 1 ml of 60% Optipreptm solution. This solution was freshly prepared and stored on ice. • 30% (vol/vol) Optipreptm solution: 1.5 ml of OD2 was mixed with 1.5 ml of 60% Optipreptm solution. This solution was freshly prepared and stored on ice.
METHODS. In this section, we introduce the framework as well as the dataset used for evaluation. We use especially trained DCNN for cells degradations. The framework is depicted in Figure 1. Further details are given in the following subsections. The framework is comprehensively evaluated on the “Photovoltaic images Dataset”, a proprietary dataset collected for this work. The details of the data collection and ground truth labeling are discussed in subsec- tion 3.2.
METHODS. A search strategy was conducted in February 2013 to identify studies in which patients (adults aged 16 years or more) with PFO, suffering from TIAs, cryptogenic stroke or persistent migraine who underwent insertion of percutaneous devices for the closure of PFO compared to patients treated with standard care (i.e., medical treatment, symptomatic or secondarily preventive such as anticoagulants or fibrinolytics). The search strategy is reported in Appendix 1 – Search Strategy. The primary outcome of included studies was the effect of PFO closure devices on the recurrence of cryptogenic strokes, transient ischaemic attacks, headache or any ischaemic abnormalities leading to clinical symptoms. HTA reports, systematic reviews and comparative prospective primary studies (trials and cohort studies) in the English language with no time restrictions were included for assessment. Two authors (IA and AMo) screened potential citations for inclusion and extracted data on standardised sheets (see Appendix 2 – Data extraction sheet). Differences of opinion were resolved by discussion with a third author (TJ). Assessment of methodological quality for randomised controlled trials was carried out using criteria from the Cochrane Handbook for Systematic Reviews of Interventions[15]. Quality was assessed according to randomisation, generation of the allocation sequence, allocation concealment, blinding and reporting of adequate follow-up. The quality of non-randomised studies was assessed in relation to the presence of potential confounders using the appropriate Newcastle-Ottawa Scales (NOS)[16]. Quality was used at the analysis stage as a means of interpreting the results. Risk of bias categories were assigned on the basis of the number of NOS items judged inadequate in each study: low risk of bias - up to one inadequate item; medium risk of bias - up to three inadequate items; high risk of bias - more than three inadequate items; very high risk of bias - when there was no description of methods.
METHODS. The methodology adopted at Fiumicino Airport to monitor the most dangerous species for air navigation (Gulls, Xxxxxxxx and Lapwings) and the outcomes of the different adopted scaring devices, is based on the daily use of a field form, filled in 3-4 times per day by the trained Safety Office staff. The form presents a first general part (including data about date, name of the compilers, etc.), a meteorological section, a bird monitoring section and a scaring devices monitoring section (Fig. 1). For further details on the form, how to fill in it, etc. see Montemaggiori 1995a). The form is usually filled in during regular patrolling inspections, or whenever the staff is alerted for presence of birds. The compiled forms (1,100-I,500 per year) are then filed and analysed. During the period 1989-90, by analysing the data obtained using this methodology, it was possible to draw a complete picture of the avian community of Fiumicino Airport and the general outcome of the different scaring devices then in use (Montemaggiori 1991a, 1992). Such results permitted the elaboration of a precise strategy in order to limit the presence of birds in the airfield creating an hostile habitat, to prevent possible birdstrikes. The adopted strategy, structured in different phases, included the use of passive and active methods and the acquisition of more specific scaring Fig. 2) ices, which joined the already existing ones fully revised (Montemaggiori 1991 b, 1995b) (Tab.1, Fig.2) Passive ecological measures Active scaring devices/activities . Limit the cultivation of arable land • Avoid crops attractive to birds a• Regular car inspections of the runways (4-5 per day) Gas cannons (Purivox) . Avoid standing or exposed water • Remote-controlled sound generators (Xxxxxxx) . Dispose of all food remains • Permanent 'distress call' acoustic system (Merlaud) . Elimination of attractive trees/bushes • Mobile 'distress call' system (Merfaud) . Grass never cut below 30 cm e . Remote-controlled high frequency sound generators(Space control) Remote-controlled video system Alt the methods in use were assembled in order to obtain a unicum of many systems simultaneously active, which operate in a linked and coordinated way. This is possible also because the operative staff is constantly trained and updated (the use of many scaring methods which operate simultaneously is highly recommended; see, for example, Stenman 1990). Recently, in order to evaluate the results obtained from the elaborated s...
METHODS. We carried out a cross sectional study in March 2018 in Siena’s Hospital (Italy). The device, Illuvia® 500UV by Aerobiotix, was tested during surgical procedures. It is a mobile novel air decontamination-recirculation system unit, provided with a patented crystalline ultraviolet C (C-UVC) reactor and HEPA filter. The environmental contamination has been monitored in the following 4 phases: I) Illuvia off and OR at rest; II) Illuvia off and OR in operational; III) Illuvia on and OR in operational; IV) Illuvia off and OR in operational. We used a particle counter to measure airborne particles (0.3, 0.5, 1.0, 3.0, 5.0, 10 µm). Air samples were withdrawn in 4 spots of the OR’s periphery. Wilcoxon rank test was performed for the statistical analysis. The statistical software Stata 12 was used for the analysis, setting the significant level at p<0.05.
METHODS. Within the activities of the Italian Study Group on Hospital Hygiene (GISIO) of the Italian Society of Hygiene, Preventive Medicine and Public Health (SItI), we carried out a multicentre cross-sectional study to assess knowledge, attitude and practices on TB prevention and control among Medical, Dentistry, Nursing and other health professions’ students. Students were enrolled in the study on a voluntary basis and were administered a previously piloted structured questionnaire. Logistic regression models were applied to explore knowledge on TB prevention by selected socio-demographic variables and University-based TB prevention practice.