Experimental. INVESTIGATIVE - the use of any treatment, Service, procedure, facility, equipment, drug, device or supply (intervention) which is not determined by the Plan to be medically effective for the condition being treated. The Plan will consider an intervention to be Experimental/Investigative if:
Experimental. Shall be defined as all procedures and treatments not covered under the Medicare Program (Title XVlll of Social Security Act of 1965, as amended), unless otherwise specifically included or excluded under this Agreement.
Experimental. Materials and reagents 2,2´-azobispyridine (apy), Ru(tpy)Cl3, [Ru(apy)(tpy)Cl](ClO4), [Ru(apy)(tpy)(H2O)](ClO4)2·2H2O and [Ru(apy)(tpy)(CH3CN)](ClO4)2 were synthesized according to the literature methods.21-23 LiCl, NaClO4 (both Merck), NaClO, AgNO3 (both Acros), tpy (Aldrich), RuCl3·3H2O (Johnson & Matthey), and 9-EtGua (Sigma) were used as supplied. All other chemicals and solvents were reagent grade commercial materials and used as received. Physical measurements C, H and N determinations were performed on a Perkin Elmer 2400 Series II analyzer. Mass spectra were obtained with a Finnigan Aqa mass spectrometer equipped with an electrospray ionization source (ESI). FTIR spectra were obtained on a Perkin Elmer Paragon 1000 FTIR spectrophotometer equipped with a Golden Gate ATR device, using the diffuse reflectance technique (res. 4 cm-1). NMR spectra were recorded on a Bruker DPX-300 spectrometer operating at a frequency of 300 MHz, at a temperature of 310 K; on a Bruker Avance-400, at a frequency of 400 MHz and 328 K, and on a Bruker DRX-500 spectrometer operating at a frequency of 500 MHz, at a variable temperature. Chemical shifts were calibrated against tetramethylsilane (TMS). [Ru(apy)(tpy)(9-EtGua)]2+ titration The pH titrations were carried out at 310 K in D2O, by adjustments with DCl and NaOD without the use of any buffer. The pH values were not corrected for the H/D isotope effect. The pH meter was calibrated with Fisher certified buffer solutions of pH 4.00, 7.00 and 10.00. Synthesis and characterization of [Ru(apy)(tpy)(9-EtGua)](ClO4)2 [Ru(apy)(tpy)(H2O)](ClO4)2·2H2O (15 mg, 0.019 mmol) and 9-EtGua (4 mg, 0.022 mmol) were vigorously refluxed in 5 mL EtOH abs for 24 hours. The mixture was left to cool down to r.t. The product was collected by filtration, washed with a small amount (about 2 mL) of ice-cold water and ether and dried in vacuo over silica (yield 82%). C32H28N12O9Cl2Ru (%) calcd C, 42.9; H, 3.1; N, 18.7. Found: C, 42.7; H, 2.7; N, 18.8. ESI- MS: m/z 697.1 ([Ru(apy)(tpy)(9-EtGua - H)]+); 348.7 ([Ru(apy)(tpy)(9-EtGua)]2+). 1H NMR (300 MHz, D2O, 310 K): δ (ppm)= 9.21 (d, 1H, 5.20 Hz); 8.92 (d, 1H, 8.22 Hz); 8.48 (t, 1H, 8.00 Hz); 8.37 (m, 3H); 8.20 (t, 1H, 8.06 Hz); 8.11 (m, 3H); 7.92 (d, 1H, 4.99 Hz); 7.64 (m, 3H); 7.41 (dd, 2H, J1 = 8.70 Hz, J2 = 14.92 Hz); 7.30 (dd, 1H, J1 = 4.28 Hz, J2 = 6.86 Hz); 6.81 (s, 1H); 6.52 (d, 1H, 7.98 Hz); 3.83 (dd, 2H, J1 = 7.21 Hz, J2 = 14.47 Hz);
Experimental. The Seebeck coefficient was measured using a home-made sample holder built on a PPMS puck. It consists of two copper blocks separated by a thermal insulator plastic. The copper has a high thermal conductance so the blocks are at a uniform temperature while a temperature gradient is produced be- tween them. A small heater (maximum power of 5 W) is installed in the upper block. Its temperature is measured with a Pt-100 resistor and controlled with an external temperature controller. The temperature of the lower block is controlled by the set point of the PPMS, but the temperature was separately measured by a second Pt-100 resistor. The whole setup is covered with a stainless steal cup that isolates the sample holder to help stabilize the tem- perature gradient. The measurements were done in a relatively low vacuum of 10 mTorr. A schematic of the sample holder is given in Fig.6.1 The samples consisted of thin films, mostly on sapphire substrates, with an area of 10 × 10 mm2. CrO2 films were deposited by Chemical Vapor Deposition (CVD) on both isostructural TiO2(100) and sapphire (1000) sub- strates. CrO2 films deposit epitaxially on TiO2 in the form of rectangular grains aligned along c-axis but on sapphire the grains are aligned with six fold rotational symmetry coming from the hexagonal structure of the substrate, as detailed in Chapter 3. The Py thin films were deposited using dc sputter- ing in a UHV sputtering system, with a base pressure of 10−9 mbar, the Co films were deposited in Z-400 an RF sputtering system with base pressure of 10−6 mbar. Both Py and Co were deposited on sapphire substrates because of its better thermal conductivity. The Seebeck coefficient was recorded with reference to copper since Cu wires were connected at both ends of the film via pressed Indium. The po- tential difference was probed using a Nanovoltmeter (Xxxxxxxx 2018) in an open circuit geometry (J = 0). A dynamic technique was utilized to measure TEM as function of temperature in which the temperature difference between hot and cold point was always 5 K, while the temperature of the cold point was increased by 10 K in each step. In this way hot point and cold point interchanged in each step between the temperature range of 100 - 400 K [80]. To check the setup, TEP was measured for nonmagnetic Cu, Au, and Pt with reference to Cu. In principal, it should give a zero TEP on a Cu thin film, but we measured around 2.5 µV at temperature difference of 45 K with the hot terminal b...
Experimental. PLAN The experimental details that follow are approximate and may be changed upon mutual agreement of the NCI and Kite. Any change in the scope of this CRADA will be by mutual consent and written Amendment to the CRADA. […***…]. […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 28 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 29 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 30 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 31 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 32 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 33 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 34 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH […***…]. DESCRIPTION OF THE CONTRIBUTIONS AND RESPONSIBILITIES OF THE PARTIES Surgery Branch, NCI — […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 35 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS CLINICAL RESEARCH — […***…]. Kite Pharma, Inc. — […***…]. NCI Surgery Branch and Kite Pharma, Inc. — […***…]. ***Confidential Treatment Requested PHS ICT-CRADA Case Ref. No. MODEL ADOPTED June 18, 2009 Page 36 of 52 Confidential PUBLIC HEALTH SERVICE COOPERATIVE RESEARCH AND DEVELOPMENT AGREEMENT FOR INTRAMURAL-PHS ...
Experimental. 2.1 High pressure laminar flow reactor The DTU high pressure flow reactor is designed to operate at pressures up to 100 bar. A brief description is given here as the setup is described in detail elsewhere [21, 38, 39]. The reactor is a 154 xx xxxx quartz tube with 8 mm ID (inner diameter) and 10 mm OD (outer diameter). A steel container surrounds the quartz tube and the cavity between the steel container and quartz tube is pressurized to form a pressure shell preventing high pressure gradients over the quartz tube. A moveable thermostat is placed inside the steel container to estimate the temperature inside the reactor. Heating trace (∼ 373 K) is used downstream to prevent product condensation. High pressure mass flow controllers are used to control the feed of the reactant gasses (ammonia, heptane and O2) and nitrogen (for dilution). Gas bottles from Air Liquid with certified concentrations of ±2% were used. The total mass flow was maintained around 1500 Nml/min, allowing a good plug flow approximation in the laminar flow regime [38]. Temperature profiles in the reactor were measured at inert conditions. We define the isothermal zone to be the length of the reactor where the temperature is within ±6 K. The isothermal zone was measured to be 45-48 cm at 21 bar and 39-45 cm at 100 bar, corresponding to residence times of 6-10 s at 21 bar and 28-57 s at 100 bar, decreasing with temperature. The downstream was analysed for products using a Trace 1300/1310 gas chromatograph (GC) from Thermo Xxxxxx for hydrocarbons, O2, CO and CO2, and ammonia levels were measured using either an FTIR analyser (MKS multigas 2030) or a dedicated NH3 analyzer (ABB AO2020). The GC uses a Flame Ionization Detector (FID) with a capillary column Rtx-5, and a Thermal Conductivity Detector (TCD) with three micro packed columns; Rt-XLSulfur, Molsieve-5A, and HS-N (HayeSep). For every measuring temperature, two to five GC samples were collected to determine the species mole fractions with a measuring uncertainty within 10%. The GC was calibrated for heptane, butane, iso-butene, propane, propene, ethane, ethene, methane, methanol, acetaldehyde, CO, CO2, and O2.
Experimental. Development personnel engaged in work of a developmental or experimental nature may perform manual work provided that such work does not deprive bargaining unit employees of work normally done by bargaining unit employees.
Experimental. The preparation of uniformly labeled self-aggregated Chl a/H2O has been described before [1]. The measurements were performed with a DSX-750 spectrometer and using a 4 mm triple resonance probe (Bruker, Germany), operating at a temperature of 277 K. The spinning frequency was kept constant within a few Hertz. During the 13C evolution intervals, heteronuclear TPPM decoupling [16] was applied with pulses of 7.3 μs and a phase modulation of 15°, using a rf nutation frequency of 66 kHz. Phase sensitive detection in the t1 dimension was achieved with a TPPI scheme [17].
Experimental. INVESTIGATIONAL: A drug, device, or dental treatment or procedure is experimentalƒinvestigational if:
Experimental. 3. Emergency situations when it has been determined that bargaining unit employees are not available.
