December 2000. 61.80524306 May 2002............ 21.34207704 August 1999......... 95.60538313 January 2001........ 59.
December 2000. Yeast 2 hybrid screen - isolation of full length clones of interacting partners, sequencing, subcloning into expression vectors. Expression and purification - recombinant 6XHis-B55 fusion proteins. Recombinant protein production at Silenus. PAbs - begin immunizing rabbits with recombinant B55. Adenoviral expression - production of B55 adenoviral stocks in 293A cells. In vitro functional studies with B55 Adenovirus. Localization studies by immunohistochemistry. Co-expression and co-immunoprecipitation - B55 and the candidate interacting partners. Signaling pathway - set up assays to analyze downstream signaling events. GST-pull down assays with C- and N-terminal recombinant B55 fragments - identify other strongly interacting proteins. A second Y2H screen to isolate other interacting clones from human liver cDNA library. Cloning and expression of B55 promoter. Studies with Biacore - fractionation of tissue homogenates by chromatography methods - test for interaction with immobilized B55. To further characterize the B55 interacting partners and their functional roles. Continue with B55 promoter studies. Set up high through put assays for drug screening based on either B55 promoter driven reporter activity or interaction between B55 and one of the interacting partners. Preliminary ICV infusion studies with chemically synthesized peptide. Immunization of rabbits with KLH-H24 conjugates. Further ICV infusion studies to analyze the dose dependent effects. ICV infusion of A, B and C animals - to study the differences in response to treatments with peptide. To analyze the test bleeds from rabbits for effective immune response.
December 2000. In vivo functional studies with best performing antisense oligos - monitor changes in [*] levels. Complete in vitro studies with [*]. Construct recombinant [*] Adenovirus and prepare viral stocks. Adenoviral mediated expression of [*] - in vitro functional studies in cell culture. In vivo functional studies - IP and IM delivery of Adenoviral [*]. Continue with in vivo functional studies. If a physiological relationship between changes in [*] and [*] levels to the diabetic or obese state of the animals is once established, methods to modulate endogenous levels of [*] activity will be investigated. [*] - Research Proposal Increased expression of [*] in hypothalamus is found to be associated with increased food intake and increase in body weight.
December 2000. The parties to the Property Management Joint Venture did not reach an agreement before 31 December 2000. No formal and written amendment to the Property Management Joint Venture has been executed to extend the time limit granted to reach a final agreement on the terms still to be agreed. As a result of the above and according to the latest discussions between GFF and Bourdais, GFF and Bourdais have agreed on the sale of SPGI and Xxxxxx & Bourdais to GFF. GFF and Bourdais intend to enter into new discussions as soon as possible with the Buyer in view of agreeing on the terms and conditions of a new property management joint venture agreement.
December 2000. If Triton has taken delivery 80% of the units within the specified period, Xxxxxxx shall grant a three month (3) grace period on the balance of Systems. Cancellation cost of $23,000 per system against minimum quantity Order of 200 in FY 2001. Payable on 30
December 2000. The above sums will be first utilised first in paying off the outstanding interest, with the balance being applied to pay off part of the principal. On or before 29 December 2000, it is agreed that representatives of Gemini and Carrier User will meet to seek to agree a mutually acceptable accelerated repayment schedule in respect of the balance of the Outstanding Sums. For the avoidance of doubt:
December 2000. This Agreement shall automatically renew thereafter in one (1) year increments until terminated pursuant to Article 9.2.
December 2000. General Services attempts to negotiate a volume purchase agreement with Oracle, but these negotiations are unsuccessful. According to DOIT, the enterprise work group—comprised of members of DOIT, the state data centers, and occasionally attended by other state agencies, including General Services and Finance—reaches a consensus that the State leverage its purchasing power for Oracle and Computer Associates products, both widely used in state and local government, by purchasing at a volume discount rate. The work group verbally recommends that the State employ a more beneficial contracting method, but does not specify a type of purchasing vehicle. Logicon makes the first of several presentations to DOIT and other state agencies regarding enterprise software solutions and the relative merits of various acquisition methods including ELAs. DOIT sends out its survey to all state, city, and county chief information offiicers, asking for information on their usage of all Oracle products and possible interest and participation in a statewide, pooled acquisition of Oracle software. Logicon presents its proposal on the Oracle software cost reduction initiative. Initially it estimates savings of over $114 million could result from an ELA. Xxxxxxx states that a contract must be signed by May 31, 2001. This is the first Logicon presentation attended by Finance's Technology Investment Review Unit (TIRU). DOIT issues a management memo on its IT acquisition policy, focusing on IT acquisition planning and oversight processes. The policy highlights the importance of asset management strategies to take full advantage of savings from enterprise volume purchase agreements. TIRU forwards its concerns about the ELA to the directors of Finance and DOIT. These concerns include: • None of the assumptions or cost savings projections are validated. • Entering into the contract may create a de facto standard. • A cost allocation model has not been developed. • Logicon's contract model, taken from a federal model, may not be appropriate for California. • A process must be created for the ELA and someone must be responsible and take ownership of the proposal. DOIT forwards TIRU's concerns to Logicon to address. • Logicon submits a draft contract to General Services. • Logicon provides copies of purchase orders of the State's historical spending on Oracle database licenses and maintenance to TIRU, DOIT, and General Services. • General Services asks DOIT to review the purchase orders and ...
December 2000. During the visit of Xx. Xxxxxxxx, Mr. xx Xxxxxx expressed the Commission’s satisfaction with progress in economic stabilisation and reform achieved so far in the former Yugoslav Republic of Macedonia and welcomed the authorities’ determination to further stimulate market forces. He also noted that since the independence of the country, substantial progress has been made with democratisation and the development of good-neighbourly relations and regional co-operation in south-eastern Europe. While macro-financial assistance will help the country with pursuing its stability-oriented budgetary and monetary policy, EIB lending is expected to help the country to develop more intensively its transport infrastructure. Though a variety of instruments the EU is therefore contributing to strengthening domestic and foreign direct investment, in particular from the EU, stimulate growth and reduce unemployment on former Yugoslav Republic of Macedonia.
December 2000. Article 1 - Scope
